Rapid Characterization of Microorganisms by Mass Spectrometry

This book contains chapters from participants in a recent symposium, organized by the Division of Analytical Chemistry during the ACS National Meeting in Washington DC in August, 2009, as well as several other active researchers in the field. The book covers aspects of mass spectrometry (MS) applications for microorganism characterization in several fields: biodefense, clinical diagnostics, food safety, environmental monitoring, and chemotaxonomy/biosystematics. The diverse list of contributors - from academia, government as well as industry present multi-faceted and broad perspectives on the subject to be presented.1. RAPID CHARACTERIZATION OF MICROORGANISMS BY MASS SPECTROMETRY: AN OVERVIEW; CATHERINE FENSELAU AND PLAMEN DEMIREV; 2. RAPID SAMPLE PREPARATION FOR MICROORGANISM ANALYSIS BY MASS SPECTROMETRY; FRANCO BASILE; 3. DISCRIMINATION OF FUNGI BY MALDI-TOF MASS SPECTROMETRY; JUSTIN M. HETTICK, BRETT J. GREEN, AMANDA D. BUSKIRK, JAMES E. SLAVEN, MICHAEL L. KASHON, AND DONALD H. BEEZHOLD; 4. FUNGAL METABOLITES FOR MICROORGANISM CLASSIFICATION BY MASS SPECTROMETRY; VLADIMIR HAVLICEK AND KAREL LEMR; 5. RAPID PROFILING OF RECOMBINANT PROTEIN EXPRESSION FROM CRUDE CELL CULTURES BY MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY (MALDI-MS); SCOTT C. RUSSELL; 6. DETECTION, DIFFERENTIATION AND SUBTYPING OF BOTULINUM NEUROTOXINS IN CLINICAL SAMPLES WITH MASS SPECTROMETRY; JOHN R. BARR, SUZANNE R. KALB, AND JAMES L. PIRKLE; 7. RAPID IDENTIFICATION OF FOOD-BORNE PATHOGENS BY TOP-DOWN PROTEOMICS USING MALDI-TOF/TOF MASS SPECTROMETRY; CLIFTON K. FAGERQUIST; 8. TOP-DOWN PROTEIN ANALYSIS AND PHYLOGENETIC CHARACTERIZATION OF UNSEQUENCED BACTERIA; NATHAN J. EDWARDS, COLIN WYNNE, AVANTIKA DHABARIA, AND CATHERINE FENSELAU; 9. MATRIX ASSISTED LASER DESORPTION IONIZATION ION MOBILITY TIME-OF-FLIGHT MASS SPECTROMETRY OF BACTERIA; JUANEKA M. HAYES, LOUIS C. ANDERSON, J. ALBERT SCHULTZ, MICHAEL UGAROV, THOMAS F. EGAN, ERNEST K. LEWIS, VIRGINIA WOMACK, AMINA S. WOODS, SHELLEY N. JACKSON, ROBERT H. HAUGE, CARTER KITTRELL, STEVE RIPLEY, AND; KERMIT K. MURRAY; 10. SINGLE-PARTICLE AEROSOL MASS SPECTROMETRY (SPAMS) FOR HIGH-THROUGHPUT AND RAPID ANALYSIS OF BIOLOGICAL AEROSOLS AND SINGLE CELLS; MATTHIAS FRANK, ERIC E. GARD, HERBERT J. TOBIAS, KRISTL L. ADAMS, MICHAEL J. BOGAN, KEITH R. COFFEE, GEORGE R. FARQUAR, DAVID P. FERGENSON, SUE I. MARTIN, MAURICE PITESKY, VINCENT J. RIOT, ABNEESH SRIVASTAVA, PAUL T. STEELE, AND AUDREY M. WILLIAMS; 11. RAPID DETECTION AND IDENTIFICATION OF AEROSOLIZED BIOLOGICAL MATERIALS, TOXINS, AND MICROORGANISMS BY AN AP-MALDI-MS-BASED SYSTEM; BERK OKTEM, APPAVU K. SUNDARAM, JANE RAZUMOVSKAYA, SESHU K. GUDLAVALLETI, THOMAS D. SAUL, AND VLADIMIR M. DOROSHENKO; 12. MALDI MASS SPECTROMETRY FOR RAPID DETECTION AND CHARACTERIZATION OF BIOLOGICAL THREATS; NATHAN A. HAGAN, JEFFREY S. LIN, MIQUEL D. ANTOINE, TIMOTHY J. CORNISH, RACHEL S. QUIZON, BERNARD F. COLLINS, ANDREW B. FELDMAN, AND PLAMEN A. DEMIREV; EDITORS' BIOGRAPHIES; AUTHOR INDEX; SUBJECT INDEX