Handbook of Phycological Methods

The contributors include some of the most eminent specialists in the field of phycology. This volume focuses on cytological and developmental methods, each method already has been applied successfully to algae, and practical examples are given.Editor's preface; Introduction; Part I. Experimental Algal Systems and Techniques: 1. Control of morphogenesis in Micrasterias Oswald Kiermayer; 2. Control of development in Scenedesmus Francis R. Trainor; 3. Mating induction in Oedogonium Gerry J. C. Hill; 4. Gamete induction and flagellar adhesion in Chlamydomonas reinhardi William J. Snell; 5. Acetabularia: techniques for study of nucleo-cytoplasmic interrelationships Sigrid Berger and Hans-Georg Schweiger; 6. Gamete release, fertilization, and embryogenesis in the Fucales Ralph S. Quatrano; 7. Hybridization and genetics of brown algae Alan R. Polanshek and John A. West; 9. Development in red algae: elongation and cell fusion Susan D. Waaland; 10. Production and selection of mutants in blue-green algae Catherine L. R. Stevens and S. Edwards Stevens, Jr.; 11. Euglena: mutations, chloroplast 'bleaching', and differentiation Harvard Lyman and Karen Traverse; 12. Crpthecodinium: sexual reproduction and mutagenesis Robert C. Tuttle; 13. Protoplast and spheroplast production Marina Adamich and Barbara B. Hemmingsen; 14. Cytoplasts from coenocyic algal cells Yolande Kersey; 15. Cytoplasmic streaming and membrane phenomena in cells of Characeae Masashi Tazawa; 16. Microbeam irradiation in Mougeotia Wolfgang Haupt; 17. Phototropism: determination of an action spectrum in a tip-growing cell Hironao Kataoka; 18. Chromatic adaption in Fremyella diplosiphon and morphogenetic changes John F. Haury; 19. How to detect the presence of a circadian rhythm Beatrice M. Sweeney; Part II. Light and Electron Microscopy: Preparative Methods: 20. Photomicrography and special microscopic techniques J. Robert Waaland; 21. Polarized light, interference, and differential interference (Nomarski) optics Paul B. Green; 22. Preparation of algae for light microscopy Margaret E. McCully, Lynda J. Goff and Patricia C. Adshead; 23. Fixation, embedding, sectioning, and staining of algae for electron microscopy Bernhard E. F. Reimann, Eleanor L. Duke and Gary L. Floyd; 24. Cytochemical localization Richard N. Trelease; 25. Quantitative cytochemical measurement of DNA in Eudorina (Chlorophyceae) C. Lindley Kemp and K. K. Nair; 26. Autoradiography for light and electron microscopy Ruth E. Schmitter; 27. Immunochemistry: labeled antibodies Esther L. McCandless, Elizabeth Gordon-Mills and Valerie Vreeland; 28. Freeze-fracture and freeze-etch techniques L. Andrew Staehelin; 29. Preparation of algae for scanning electron microscopy Jeremy D. Pickett-Heaps; 30. Replica production and negative staining Elisabeth Gantt; 31. Preparation of shadow-cast whole mounts Ojvind Moestrup and Helge A. Thonsen; 32. Stereology: quantitative electron microscopic analysis Wayne R. Fagerberg; Part III. Appendixes: 33. Culture collections; 34. List of suppliers; Part IV. Indexes: 35. Subject; 36. Author; 37. Taxonomic.