Quantitative Biomedical Optics

This is the textbook and reference resource that instructors, students, and researchers in biomedical optics have been waiting for. Comprehensive and up to date, it covers a broad range of areas in biomedical optics, from light interactions at the single-photon and single-biomolecule levels, to the diffusion regime of light propagation in tissue. Subjects covered include spectroscopic techniques (fluorescence, Raman, infrared, near-infrared, and elastic scattering), imaging techniques (diffuse optical tomography, photoacoustic imaging, several forms of modern microscopy, and optical coherence tomography), and laser-tissue interactions, including optical tweezers. Topics are developed from the fundamental principles of physical science, with intuitive explanations, while rigorous mathematical formalisms of theoretical treatments are also provided. For each technique, descriptions of relevant instrumentation and examples of biomedical applications are outlined, and each chapter benefits from references and suggested resources for further reading, and exercise problems with answers to selected problems. 'Bigio and Fantini provide a long-needed introduction to the field of biomedical optics and biophotonics, adding spice to the presentation of the basics with historical and etymological gems. The conversational tone of the book is very welcome, and allows room for the clear explanation of subtleties not always clarified in other discussions. The book is a wonderful introduction to the field. It balances rigor with readability. Bravo!' Steven L. Jacques, Oregon Health Sciences University 'This book about biomedical optics provides a remarkably comprehensive introduction to the field. The text is carefully and affectionately developed with quantitative rigor, and it is written in a clear, easy-to-understand style that helps students develop intuition. The subject matter covers basics of linear and nonlinear optical spectroscopy, static and dynamic light scattering and more advanced topics such as light transport through highly scattering tissues, acousto-optics and opto-acoustics, and imaging from microscopy to tomography. The book should prove useful as a textbook for courses targeting both advanced undergraduates and graduate students in science, engineering and medicine. It will also be a valuable reference for researchers working at the frontiers of knowledge.' Arjun G. Yodh, University of Pennsylvania 'Bigio and Fantini's comprehensive text on biomedical optics provides a wonderful blend of accessible theory and practical guidance relevant to the design and application of biomedical optical systems. It should be required reading for all graduate students working in this area.' Rebecca Richards-Kortum, Rice University, Houston1. Nomenclature; 2. Overview of tissue optical properties; 3. Introduction to biomedical statistics for diagnostic applications; 4. General concepts of tissue spectroscopy and instrumentation; 5. Autofluorescence spectroscopy and reporter fluorescence; 6. Raman and infrared spectroscopy of vibrational modes; 7. Elastic and quasi-elastic scattering from cells and small structures; 8. Diffuse reflectance spectroscopy at small source-detector separations; 9. Transport theory and the diffusion equation; 10. Continuous-wave methods for tissue spectroscopy; 11. Time-domain methods for tissue spectroscopy in the diffusion regime; 12. Frequency-domain methods for tissue spectroscopy in the diffusion regime; 13. Instrumentation and experimental methods for diffuse tissue spectroscopy; 14. Diffuse optical imaging and tomography; 15. In vivo applications of diffuse optical spectroscopy and imaging; 16. Combining light and ultrasound: acousto-optics and opto-acoustics; 17. Modern optical microscopy for biomedical applications; 18. Optical coherence tomography; 19. Optical tweezers and laser-tissue interactions.